| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Preparation Note | Aprotinin is freely soluble in water (>10 mg/ml) and in aqueous buffers of low ionic strengths. Dilute solutions are generally less stable than concentrated ones. Solution stability is pH dependent; a range of 1-12 can be tolerated. Repeated freeze-thaw cycles should be avoided. Due to its compact tertiary structure, aprotinin is relatively stable against denaturation due to high temperature, pH extremes, organic solvents, or proteolytic degradation (only thermolysin has been found capable of degrading aprotinin after heating to 60-80 °C). The high basicity of aprotinin causes it to adhere to commonly used dialysis tubing and even gel filtration matrices, but the use of acetylated materials and concentrated salt solutions (=0.1 M NaCl in buffer) minimizes the problem. Sterilization may be achieved by filtration through a 0.2 μm filter. |
| | Contains no animal-derived components or impurities, and is manufactured by transient expression of the aprotinin message in RNA (+)-strand tobacco mosaic virus vectors propagated in non-transgenic Nicotiana plants. This is a recombinant form of the native, bovine-sequence aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. Unlike animal-derived aprotinin, this product is isolated and purified from plant tissue by proprietary methods. |
| Unit Definition | One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C. |
